Recombinant Adipophilin Antibody / ADRP / Perilipin 2 / PLIN2

SKU: orb2641442

Description

Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.

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Tested Applications	IHC-P
Dilution Range	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Reactivity	Human
Application Notes	Titering of the recombinant Adipophilin antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	ADFP/2755R
Immunogen	A portion of amin acids 249-376 from the human protein were used as the immunogen for this recombinant Adipophilin antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant Adipophilin Antibody / ADRP / Perilipin 2 / PLIN2

IHC testing of FFPE human adrenal tissue with recombinant Adipophilin antibody (clone ADFP/2755R). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Adipophilin antibody (clone ADFP/2755R) as confirmation of integrity and purity.

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UniProt

Q99541

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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