Recombinant Adipophilin Antibody / ADRP / Perilipin 2 / PLIN2

SKU: orb606688

Description

Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.

Research Area

Epigenetics

Images & Validation

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Tested Applications	ELISA, IHC-P, WB
Dilution Range	ELISA (order BSA/sodium azide-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Titering of the recombinant Adipophilin antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	ADFP/2755R
Immunogen	A portion of amin acids 249-376 from the human protein were used as the immunogen for this recombinant Adipophilin antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant Adipophilin Antibody / ADRP / Perilipin 2 / PLIN2

IHC testing of FFPE human adrenal tissue with recombinant Adipophilin antibody (clone ADFP/2755R). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.

Western blot testing of human liver lysate with recombinant Adipophilin antibody. Predicted molecular weight ~48 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant Adipophilin antibody (clone ADFP/2755R) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q99541

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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