AFP Rabbit Polyclonal Antibody

SKU: orb1181

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Description

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human AFP (501-609/609aa)
Target	AFP
Molecular Weight	65 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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alpha Fetoprotein; alpha-fetoprotein; alpha 1 fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.

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Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AFP) Polyclonal Antibody, Unconjugated (orb1181) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HepG2 Cell (Human) Lysate at 40 ug, Primary: Anti-AFP (orb1181) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.

Quick Database Links

Gene Symbol

AFP

UniProt

P02771

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Mandon, Céline A. et al. Polymer adhesive surface as flexible generic platform for multiplexed assays biochip production Biosens Bioelectron, 39, 37-43 (2013)

PubMed: 22795528