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AIF/Iba1 Antibody
SKU: orb1294405
Description
Images & Validation
−Item 1 of 8
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:100-1:300 Immunohistochemistry (Paraffin) 1:100-1:300 Immunohistochemistry (Frozen) 1:100-1:300 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
| Target | AIF / Iba1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
Quality Guarantee
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Frozen sectioned for immunohistochemical analysis. Sample: Frozen sectioned Rat brain. Green: Iba1 antibody (orb1294405), Red: TUJ1 antibody, Blue: DAPI.
Iba1/AIF1 antibody - at 1/500 dilution, Lysates at 45 µg per lane, This blot was produced using a 15% SDS-PAGE. PVDF membrane was then blocked with 3% BSA for an hour before being incubated with orb1294405 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded Rat brain tissue labeling Iba1 with orb1294405 at 1/200. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical of frozen sections. Sample: mouse cerebellum. Green: Iba1 antibody (orb1294405): 1-200, Anti-rabbit 488: 1-500. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical of frozen sections. Sample: mouse hippocampus. Green: Iba1 antibody (orb1294405): 1-200, Anti-rabbit 488: 1-500. DAPI (blue) was used as the nuclear counter stain.
Quick Database Links
Gene Symbol
AIF / Iba1
Documents Download
Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
AIF/Iba1 Antibody (orb1294405)
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