ALOX12 Rabbit Polyclonal Antibody

SKU: orb5649

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Description

Research Area

Metabolism Research

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	WB=1:500-2000
Reactivity	Human, Mouse
Predicted Reactivity	Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human 12 Lipoxygenase (101-200/663aa)
Target	ALOX12B
Molecular Weight	76 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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12-LOX; 12S-LOX; LOG12; LOX12_HUMAN; ALOX12; Arachidonate (12S)-lipoxygenase (12S-LOX | 12S-lipoxygenase); Arachidonate (15S)-lipoxygenase; Linoleate (13S)-lipoxygenase; Lipoxin synthase 12-LO; Platelet-type lipoxygenase 12; 1.13.11.-; 12LO;

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Sample: Lane 1: Esophagus (Mouse) Lysate at 40 ug, Lane 2: A431 (Human) Cell Lysate at 30 ug, Lane 3: Hela (Human) Cell Lysate at 30 ug, Lane 4: A549 (Human) Cell Lysate at 30 ug, Lane 5: 293T (Human) Cell Lysate at 30 ug, Lane 6: U937 (Human) Cell Lysate at 30 ug, Lane 7: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-ALOX12 (orb5649) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 76 kD, Observed band size: 76 kD.

Sample: Raw264.7 Cell Lysate at 40 ug, A431 Cell Lysate at 40 ug, Primary: Anti-ALOX12 (orb5649) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 73 kD, Observed band size: 73 kD.

Quick Database Links

Gene Symbol

ALOX12B

UniProt

P18054

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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