AKT1 Rabbit Monoclonal Antibody

SKU: orb546987

Description

Anti-AKT1 Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Metabolism Research, Protein Biochemistry, Signal Transduction

Images & Validation

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Item 1 of 5

Tested Applications	FC, ICC, IF, IHC, IP, WB
Dilution Range	WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 IP 1:50 FC 1:50
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone No.	EBG-1
Immunogen	A synthesized peptide derived from human AKT1 Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
Target	RAC-alpha serine/threonine-protein kinase
Molecular Weight	56 kDa
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml rAlbumin.
Concentration	0.5mg/ml
Disclaimer	For research use only

Alternative Names

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Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3; APRF

Quality Guarantee

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WB analysis of AKT1 expression in MCF-7 Cell.

WB analysis of NOTCH1 expression in (1) HepG2 Cell; (2) A549 Cell; (3) HeLa Cell; (4) Raji Cell; (5) MCF-7 Cell; (6) SiHa Cell; (7) 293T Cell.

WB analysis of NOTCH1 expression in (1) Rat liver Tissue; (2) Rat brain Tissue; (3) Rat C6 Cell; (4) Rat RH35 Cell; (5) Mouse liver Tissue; (6) Mouse brain Tissue; (7) Mouse Neuro-2a Cell; (8) Mouse HEPA1-6 Cell.

Immunofluorescent analysis of HeLa cells, using AKT1 Antibody.

Immunohistochemical analysis of paraffin-embedded human colon, using AKT1 Antibody.

Quick Database Links

Gene Symbol

RAC-alpha serine/threonine-protein kinase

UniProt

P31749

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ICC

Immunocytochemistry

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Immunoprecipitation

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