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B7-2 Antibody
SKU: orb757407
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, FC |
|---|---|
| Dilution range | ELISA 1/5000-10000|Flow Cyt 1/100 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | DM85 |
| Target | B7-2 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Disclaimer | For research use only |
Alternative Names
−CD86, B7-2, B70, CD28LG2, LAB72, MGC34413
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ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-2 protein, mFc-His tagged protein orb689395 can bind Rabbit anti-B7-2 monoclonal antibody (clone: DM85) in a linear range of 1-25 ng/ml.
Expi 293 cell line transfected with irrelevant protein (A) and human B7-2 (B) were surface stained with Rabbit anti-B7-2 monoclonal antibody 1 μg/ml (clone: DM85) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Flow cytometry analysis of antigen binding of rabbit anti-human B7-2 mAb (orb757407). (A) orb757407 does not bind to 293T cells that do not express B7-2. (B) A clear peak shift of orb757407 was seen compared to the control when incubated with B7-2-expressing Daudi cells, indicating strong binding of orb757407 to B7-2. Antibodies were incubated at 5 μg/mL
Flow cytometry analysis of antigen binding of rabbit anti-human B7-2 mAb (orb757407). (A) orb757407 does not bind to 293T cells that do not express B7-2. (B) A clear peak shift of orb757407 was seen compared to the control when incubated with B7-2-expressing Raji cells, indicating strong binding of orb757407 to B7-2. Antibodies were incubated at 5 μg/mL
Quick Database Links
Gene Symbol
B7-2
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
B7-2 Antibody (orb757407)
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