beta-Catenin Antibody

SKU: orb43778

Description

Mouse Monoclonal to beta Catenin.

Research Area

Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways, Stem Cells, Wnt

Images & Validation

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Item 1 of 3

Tested Applications	FC, ICC, IP, WB
Reactivity	Hamster, Human, Mouse
Application Notes	Immunocytochemistry: Positive control: HT29 human colon adenocarcinoma cell line. Western blotting: Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line, KW1 murine cell line, C57MG murine cell line, 3T3 murine fibroblast cell line. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining. Read more...

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG2a
Clone No.	EM-22
Immunogen	Recombinant human beta-catenin
Target	beta-Catenin
Purification	Purified by protein-A affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CTNNB1, EVR7, MRD19

Beta catenin Rabbit Polyclonal Antibody [orb500798]

Applications:	IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep, Zebrafish
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 50 μl, 100 μl, 200 μl

Item 1 of 10
beta Catenin/CTNNB1 Rabbit Polyclonal Antibody [orb402308]
Applications:
ELISA, FC, ICC, IF, IHC, WB
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg
Item 1 of 7
beta-Catenin Antibody [orb153335]
Applications:
IF, IHC-P, WB
Reactivity:
Canine, Human, Monkey, Mouse, Rat
Species/Host:
Goat
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg

Item 1 of 4

Beta catenin Rabbit Polyclonal Antibody [orb10246]

Applications:	IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep, Zebrafish
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 50 μl, 100 μl, 200 μl

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Western blotting analysis of beta-catenin in murine 3T3 (A), C57 (B) and KW1 (C) cell lines using EM-22 antibody.

Separation of MCF-7 cells stained using anti-beta-Catenin (EM-22) purified antibody (concentration in sample 9 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Immunocytochemistry staining of beta-catenin in human colon adenocarcinoma cell line HT29 using EM-22 antibody (green). Cell nuclei visualized by DAPI (blue).

Quick Database Links

Gene Symbol

beta-Catenin

UniProt

P35222

NCBI Gene ID

1499

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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ICC

Immunocytochemistry

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Immunoprecipitation

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