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CD20 Antibody
SKU: orb1474012
Featured
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB (1/500 - 1/1000), IH (1/50 - 1/200) |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD20. The exact sequence is proprietary. |
| Purification | This antibody is purified through a protein G column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−CD20; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD20
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Western blot analysis of CD20 expression in Ramos (A) whole cell lysates. (Predicted band size: 33 kD; Observed band size: 33 kD)
Immunohistochemical analysis of CD20 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
CD20 Antibody (orb1474012)
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