CSF2Rb/CD131 Antibody

SKU: orb1806062

Description

Anti-CSF2Rb / CD131 Reference Antibody is expressed from CHO. The heavy chain type is huIgG4, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Images & Validation

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Tested Applications	ELISA, FA, FACS, In vivo
Reactivity	Human

Key Properties

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Clonality	Monoclonal
Target	CSF2Rb / CD131
Source/Expression System	CHO
Molecular Weight	144.66 kDa
Purity	>95%
Purification	Protein A
Endotoxin	<0.001 EU/ug
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100 mM Proline/Acetic acid 20mM Arginine pH 5.0
Concentration	Batch dependent
Disclaimer	For research use only

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Anti-CSF2Rb / CD131 Reference Antibody (CSL311) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-CSF2Rb / CD131 Reference Antibody (CSL311)is more than 98.34% ;determined by SEC-HPLC.

Immobilized human CSF2RB His at 2 μg/mL can bind Anti-CSF2Rb / CD131 Reference Antibody (CSL311);EC50=0.0122μg/mL.

Human CSF2RB HEK293 cells were stained with Anti-CSF2Rb / CD131 Reference Antibody (CSL311) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC114=0.049 μg/mL.

IL-3-mediated proliferation of TF-1 can be completely blocked by antibodies with an IC50 of 0.2109 nM.

GM-CSF-mediated proliferation of TF-1 can be completely blocked by antibodies with an IC50 of 4.105 nM.

Quick Database Links

Gene Symbol

CSF2Rb / CD131

UniProt

P32927

UniProt Details

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No UniProt data available

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Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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