GSK3 beta Mouse Monoclonal Antibody

SKU: orb1474223

Description

The GSK3 beta Antibody is suitable for FC, IF, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant GSK-3β protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

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Tested Applications	FC, IF, IP, WB
Dilution Range	WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), FC (1/50 - 1/100)
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant GSK-3β protein fragments expressed in E.coli.
Target	GSK3B
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Western blot analysis of GSK3 beta expression in A549 (A), Hela (B), NIH3T3 (C), rat brain (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)

Immunofluorescent analysis of GSK3 beta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

GSK3B

UniProt

P49841, P18266, Q9WV60

NCBI Gene ID

56637, 84027, 2932

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunofluorescence

Immunoprecipitation