GSK3 beta Mouse Monoclonal Antibody

SKU: orb1474238

Description

The GSK3 beta Antibody is suitable for FC, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant fragment of human GSK3B (AA: 2-159) expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

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Item 1 of 3

Tested Applications	FC, IHC, WB
Dilution Range	WB (1/500 - 1/1000), IH (1/50 - 1/100), FC (1/50 - 1/100)
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human GSK3B (AA: 2-159) expressed in E. Coli.
Target	GSK3B
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Western blot analysis of GSK3 beta expression in Hela (A), NIH3T3 (B), C6 (C), rat brain (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)

Immunohistochemical analysis of GSK3 beta staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.106). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of GSK3 beta in NIH3T3 cells (green) and negative control (red).

Quick Database Links

Gene Symbol

GSK3B

UniProt

P49841, P18266, Q9WV60

NCBI Gene ID

56637, 84027, 2932

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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