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Histone H3 (di methyl K9) HIST1H3A Rabbit Monoclonal Antibody
SKU: orb548485
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ICC, IF, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | GBI-8 |
| Immunogen | A synthesized peptide derived from human Histone H3 |
| Target | T-cell surface glycoprotein CD3 zeta chain |
| Molecular Weight | 15 kDa |
| Purification | Affinity-chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Concentration | 0.5mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ
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Histone H3 (di methyl K9) Rabbit monoclonal antibody [orb2298152]
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Rabbit
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Unconjugated
100 μl, 50 μl, 25 μl
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Western blot analysis of Histone H3 (di methyl K9) expression in HeLa cell lysate (orb548485). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # orb548485) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for HIST1H3A.
Quick Database Links
Gene Symbol
T-cell surface glycoprotein CD3 zeta chain
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ICC
Immunocytochemistry
Histone H3 (di methyl K9) HIST1H3A Rabbit Monoclonal Antibody (orb548485)
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