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CD45 Antibody (Biotin)
SKU: orb44267
Description
Images & Validation
−Item 1 of 2
| Tested Applications | FC, ICC, IHC-P, IP, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | MEM-28 |
| Immunogen | Human thymocytes and T lymphocytes. |
| Target | CD45 |
| Purification | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store at 2-8°C. Do not freeze. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−LCA, T200, LY5, B220, GP180, TPC
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Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD45 (MEM-28) biotin.
Anti-Hu CD45 Biotin (clone MEM-28) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Raji, HeLa, and HEK293T cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with biotinylated mouse IgG1 monoclonal antibody MEM-28 (1 µg/ml), followed by IRDye 800CW Streptavidin (green). Multiplex fluorescent Western blot detection was performed. CD45 molecules were detected at ~180-250 kDa in Jurkat and Raji cell lines.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
ICC
Immunocytochemistry
IP
Immunoprecipitation
CD45 Antibody (Biotin) (orb44267)
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