CD45 Antibody (Biotin)

SKU: orb2937686

Description

Images & Validation

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Item 1 of 2

Tested Applications	FC
Reactivity	Human
Application Notes	Flow cytometry: Recommended dilution: 1-5 μg/ml

Key Properties

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Clonality	Monoclonal
Isotype	Mouse IgG1 kappa
Clone No.	2D1
Immunogen	Human peripheral blood mononuclear cells
Target	CD45
Purification	Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Conjugation	Biotin

Storage & Handling

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Storage	Store at 2-8°C. Do not freeze.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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LCA, T200, LY5, B220, GP180, TPC

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Separation of human lymphocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD45 (2D1) biotin antibody (concentration in sample 1.67 µg/ml, Streptavidin APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (2D1) biotin antibody (concentration in sample 1.67 µg/ml, Streptavidin APC).

Quick Database Links

Gene Symbol

CD45

UniProt

P08575

NCBI Gene ID

5788

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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