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IL-2 Antibody (PE)
SKU: orb1742780
Description
Images & Validation
−Item 1 of 3
| Tested Applications | FC |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Isotype | Mouse IgG2b kappa |
| Clone No. | 35C3 |
| Immunogen | recombinant human IL-2 |
| Target | IL-2 |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | PE |
Storage & Handling
−| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
|---|---|
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin 2
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Flow cytometry multicolor surface staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood) and intracellular staining using anti-human IL-2 (35C3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Separation of human IL-2 positive CD3 positive lymphocytes (red-filled) from IL-2 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry intracellular staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
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IL-2 Antibody (PE) (orb1742780)
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