You have no items in your shopping cart.
Description
Research Area
Cell Biology, Disease Biomarkers, Neuroscience
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1.430555556, IHC: 0.388888889, ICC: 0.388888889 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 4D11 |
| Immunogen | Recombinant human Nestin expressed in and purifeid from E. coli. |
| Target | Nestin |
| Molecular Weight | 220-240 |
| Purification | Protein G Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS + 50% glycerol and 5 mm NaN3 |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ESTM 46 antibody, FLJ 21841 antibody, FLJ21841 antibody, Intermediate filament protein antibody, Nbla00170 antibody, nes antibody, NEST_HUMAN antibody, Nestin antibody
Similar Products
−- Item 1 of 11
Nestin Rabbit Polyclonal Antibody [orb11109]
ELISA, IHC-P, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μg - Item 1 of 6
Nestin Rabbit Polyclonal Antibody [orb251614]
ELISA, ICC, IF, IHC-P
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μg - Item 1 of 7
Nestin Antibody (S1409) [orb1931833]
FC, IF, IHC-P, WB
Human
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl - Item 1 of 3
Mouse Nestin Antibody [orb98393]
ICC, IHC-Fr, IHC-P, WB
Human, Monkey, Primate
Mouse
Monoclonal
Unconjugated
0.1 mg - Item 1 of 6
Nestin Rabbit Polyclonal Antibody [orb334607]
IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μg
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Immunolabeling of the coronal section of an embryonic day 15.5 mouse cortex showing specific labeling of Nestin (Cat. orb319583, 1:500; green). Nuclei stained with DAPI (blue).
Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (Cat. orb319583, red, 1:500) and anti-MAP2 antibody (Cat. orb319566, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
Immunostaining of cultured neonatal rat neurons and glia stained with anti-nestin antibody (Cat. orb319583, red, 1:500) and anti-vimentin antibody (Cat. orb319707, green, 1:500). The blue is Hoechst staining for nuclear DNA. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 kDa nestin doublet.
Quick Database Links
Gene Symbol
Nestin
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Nestin Antibody (orb319583)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0
Please add an antibody product to your cart first.
Usually dispatched within 5-10 working days