Nestin/NES Antibody

SKU: orb1294409

Description

Rabbit polyclonal antibody to Nestin antibody (NES)

Images & Validation

−

Item 1 of 6

Tested Applications	IF, IHC, WB
Dilution range	Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:200
Reactivity	Human, Mouse, Rat

Key Properties

−

Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / encompassing a sequence within the N-terminus region.
Target	Nestin / NES
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Anti-Nestin antibody at 1/1000 dilution, Lysates at 30 µg per lane.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294409 anti-Nestin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with orb1294409 at 1/100.

Sample: Neuron 2A, Nestin antibody (orb1294409): 1-1000.

Quick Database Links

Gene Symbol

Nestin / NES

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

Immunofluorescence

View Protocol

Find More Protocols