Phospho-Akt AKT1 Rabbit Monoclonal Antibody

SKU: orb548568

Description

Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cell Biology, Metabolism Research, Protein Biochemistry, Signal Transduction

Images & Validation

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Item 1 of 3

Tested Applications	ICC, IF, IHC, WB
Dilution Range	WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:100-1:500
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone No.	IFD-1
Immunogen	A synthesized peptide derived from human Phospho-Akt (Ser473)
Target	RAC-alpha serine/threonine-protein kinase
Molecular Weight	65 kDa
Purification	Affinity-chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml rAlbumin.
Concentration	0.5mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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RAC-alpha serine/threonine-protein kinase; 2.7.11.1; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB, RAC

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Phospho-Akt AKT1 Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-Akt (Ser473) Antibody.

Western blot analysis on 2 NIH/3T3 cell lysate treated with PDGF using Phospho-Akt (Ser473) Antibody.

Immunofluorescent analysis of NIH/3T3 cells treated with PDGF, using Phospho-Akt (Ser473) Antibody.

Quick Database Links

Gene Symbol

RAC-alpha serine/threonine-protein kinase

UniProt

P31749

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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