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VEGFA Antibody
SKU: orb1289717
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution range | WB: 1:500-1:1000, IH: 1:50-1:200, IF:IC: 1:50-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Recombinant protein corresponding to human VEGFA. The exact sequence is proprietary. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−VEGF; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF
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Western blot analysis of VEGFA expression in HEK293T (A), A549 (B), mouse liver (C) whole cell lysates. (Predicted band size: 15-27; 34-45 kD; Observed band size: 16; 34; 45 kD)
Immunofluorescent analysis of VEGFA staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
VEGFA Antibody (orb1289717)
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