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VEGFA Rabbit Polyclonal Antibody
Description
Images & Validation
−| Tested Applications | WB |
|---|---|
| Dilution range | WB=1:500-1000 |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human VEGF (27-120/232aa) |
| Target | VEGFA |
| Molecular Weight | 24 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Sample: Heart (Mouse) Lysate at 30 ug, Primary: Anti-VEGF (orb500818) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 25 kD.

Cell: Hela, Concentration: 1:100, Host/Isotype: Rabbit/IgG, Flow cytometric analysis of Rabbit antibody (orb500818) on Hela (green) compared with isotype control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody.

Sample: heart (Mouse) Lysate at 40 ug, A549 Cell (Human) Lysate at 30 ug, Primary: Anti-VEGF (orb500818) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 24 kD.

Sample: Kidney (Mouse) Lysate at 30 ug, Primary: Anti-VEGF (orb500818) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 25 kD.
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VEGFA Rabbit Polyclonal Antibody (orb500818)
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