Vinculin Rabbit Monoclonal Antibody

SKU: orb1474478

Description

The Vinculin Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant protein of human Vinculin .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

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Item 1 of 2

Tested Applications	IHC, WB
Dilution Range	WB (1/500 - 1/1000), IH (1/50 - 1/100)
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Monoclonal
Immunogen	Recombinant protein of human Vinculin
Target	VCL
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Vinculin; Metavinculin; MV

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Western blot analysis of Vinculin expression in K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates. (Predicted band size: 124 kD; Observed band size: 124 kD)

Immunohistochemical analysis of Vinculin staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.18). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

VCL

UniProt

P85972, Q64727, P18206

NCBI Gene ID

7414, 22330, 305679

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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