AX-024 hydrochloride

SKU: orb1302888

Description

AX-024 HCl is a small molecule cytokine release inhibitor that potently suppresses the production of pro-inflammatory cytokines including IL-6, TNF-α, IFN-γ, IL-10, and IL-17A. It is a valuable research tool for studying inflammatory and autoimmune diseases in both in vitro cellular assays and in vivo animal models.

Research Area

Cell Biology, Immunology & Inflammation, Neuroscience

Images & Validation

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Key Properties

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CAS Number	1704801-24-0
MW	375.87
Purity	99.90% (May vary between batches)
Formula	C₂₁H₂₃ClFNO₂
SMILES	Cl.COc1ccc2OCC(CN3CCCC3)=C(c3ccc(F)cc3)c2c1
Target	IL Receptor,Interleukin,TNF,COX,IFNAR
Solubility	DMSO:45 mg/mL (119.72 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:2 mg/mL (5.32 mM)

Bioactivity

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In Vivo	Treatment with AX-024 hydrochloride results in a significant reduction of scales and skin thickening when compared to the vehicle-treated group, demonstrating particular effectiveness in diminishing the thickening of the dermis to levels akin to those observed in mice treated with a control cream without imiquimod (IMQ). Additionally, administration of AX-024 hydrochloride notably reduces the number of airway inflammatory cells and facilitates rapid recovery from neurological impairment and weight loss in mice. By day 30, mice treated with AX-024 hydrochloride become symptom-free, contrasting sharply with vehicle-treated mice, which continue to exhibit symptoms such as ataxia and loss of the righting reflex. Read more...
In Vitro	Compound AX-024 hydrochloride exhibits over 10,000 times greater efficacy than its precursor, AX-000, in inhibiting TCR-triggered T cell proliferation, with an IC50 value of 1 nM and showing effects at concentrations as low as 1 pM. Additionally, it significantly outperforms AX-000 in suppressing cytokine release from human peripheral blood mononuclear cells activated with anti-CD3, effectively reducing the production of IL-6, TNFα, IFN-γ, IL-10, and IL-17A at a 10 nM concentration. In OT1 TCR transgenic (OT1Tg) mice with wild-type (WT) cells, AX-024 hydrochloride robustly inhibits T cell proliferation at a 0.1 nM concentration when the OT1Tg T cells are WT for the PRS mutation. Furthermore, coimmunoprecipitation experiments have demonstrated that Nck recruitment to the TCR is blocked by AX-024 hydrochloride in a concentration-dependent manner starting at 1 nM, indicating the compound's potent inhibitory action on T cell activation processes. Read more...
Cell Research	Spleen B cells from C57BL/6 mice are labeled with Cell Trace Violet and incubated for 72 hours with either anti-IgM (10 mg/mL) or anti-CD40 (5 mg/mL), supplemented with IL-4 (5 ng/mL) or LPS (2.5 mg/mL) in the presence of different concentrations of AX-024 hydrochloride. Proliferation is calculated according to the total number of cell divisions. They are for reference only. Read more...
Animal Research	Eight-week-old CD-1 mice are injected intraperitoneally with different amounts of the AX-024 hydrochloride dissolved in 0.5 mL of saline. All animals are observed Clinically for the appearance of macroscopically visible adverse reactions twice daily over 14 days, as well as immediately after AX-024 hydrochloride administration. A necropsy is carried out on each animal on day 14, and the abdominal, thoracic, and cranial cavities are examined in situ, together with their associated organs. They are for reference only. Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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inhibit, Inhibitor, IFNAR, IFN-γ, Interferon-α/β receptor, Interferon-alpha/beta receptor, Interleukin, Interleukin Related, IL-10, IL Receptor, ILReceptor, IL-6, IL-17A, AX 024, AX 024 Hydrochloride, AX 024 hydrochloride, AX024, AX-024, AX-024 HCl, AX024 Hydrochloride, AX024 hydrochloride, AX-024 Hydrochloride, TNF Receptor, TNFR, TNF-α, Tumor Necrosis Factor Receptor

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Compound Identifiers

PubChem CID

129909862 ↗

Key Properties

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No computed properties available.

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