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B7-2 Recombinant Protein
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse B7-2, Fc Tag at 5 ug/mL (100 ul/well),can bind Mouse CTLA-4, His Tag with a linear range of 0.8-12.5 ng/mL. |
| Target | B7-2 |
| Tag | Fc Tag |
| Molecular Weight | 52 kDa |
| Protein Sequence | Val 24 - Glu 245 |
| Purity | >95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human B7-2 Protein, mFc-His Tag [orb689395]
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 52.4 kDa after removal of the signal peptide.
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30 μl, 200 μl, 100 μl, 50 μlB7-2 (CD86) (NM_175862) Human Recombinant Protein [orb3036347]
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20 μg, 1 mgHuman B7-2 Protein, hFc Tag [orb689458]
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 51.5 kDa after removal of the signal peptide.
Mammalian
50 μg, 100 μg, 10 μgMouse B7-2 Protein, hFc Tag [orb1290895]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 51.4 kDa after removal of the signal peptide. The apparent molecular mass of mB7-2-hFc is approximately 55-70 kDa due to glycosylation.
Mammalian
100 μg, 50 μg, 10 μg

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Immobilized Mouse B7-2, Fc Tag at 5 ug/ml (100 ul/well), can bind Mouse CTLA-4, His Tag with a linear range of 0.8-12.5 ng/mL.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_062261.3 |
|---|
Documents Download
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Protocol Information
B7-2 Recombinant Protein (orb1227705)
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