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BAX Rabbit Polyclonal Antibody
SKU: orb1294359
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:1000-1:2000, Immunofluorescence 1:200-1:400, Immunohistochemistry (Paraffin) 1:100-1:250 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the N-terminus region. |
| Target | BAX |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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All lanes: Anti-BAX antibody at 1/1000 dilution, Lysates/proteins at 45 µg per lane. This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294359 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294359 anti-BAX antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling BAX with orb1294359 at 1/200.
Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling BAX with orb1294359 at 1/200.
Immunohistochemical analysis of paraffin embedded Human Tonsil carcinoma labeling BAX with orb1294359 at 1/200.
Quick Database Links
Gene Symbol
BAX
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
BAX Rabbit Polyclonal Antibody (orb1294359)
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