Beta-actin Antibody

SKU: orb1939450

Description

Beta-actin Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

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Item 1 of 6

Tested Applications	IHC-P, WB
Dilution Range	WB - 1:1000, IHC-P - 1:100-500
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Molecular Weight	41737 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Beta-Actin Rabbit Polyclonal Antibody (Loading Control) [orb500817]

Applications:	FC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Canine, Feline, Fish, Gallus, Guinea pig, Hamster, Insect, Porcine, Rabbit, Sheep
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 1 ml, 500 μl, 100 μl, 200 μl

Item 1 of 10
Beta Actin Antibody [orb420309]
Applications:
ELISA, IF, WB
Reactivity:
Frog, Human, Mouse
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 200 μg
Item 1 of 10
Beta Actin Antibody [orb420310]
Applications:
ELISA, IF, WB
Reactivity:
Frog, Human, Mouse
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 25 μl

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All lanes: Anti-Beta-actin Antibody at 1:2000 dilution + HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/8000 dilution.Observed band size: 42 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Beta-actin Antibody at 1:4000-1:8000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded H. spleen section using Beta-actin Antibody. diluted at 1:25 dilution. A peroxidase-conjugated goat anti-Mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded Human Ovarian cancer section using Pink1. diluted at 1:50 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysates from HepG2, HL-60 cell line (from left to right), using Beta-actin Antibody. diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary Antibody. Lysates at 20 μg per lane.

Western blot analysis of lysates from HepG2, HL-60, mouse NIH/3T3 cell line, mouse cerebellum and rat stomach tissue lysate, CHO, COS-7 cell line lysate (from left to right), using Beta-actin Antibody. diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary Antibody. Lysates at 35 μg per lane.

Quick Database Links

UniProt

P60709

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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