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Beta Tubulin 3 Rabbit Polyclonal Antibody

SKU: orb1294407

Description

Rabbit polyclonal antibody to Beta Tubulin 3

Research Area

Neuroscience

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P, WB
Dilution RangeWestern Blot 1:5000 - 1:10000, Immunofluorescence 1:200-1:500, Immunohistochemistry (Paraffin) 1:100-1:300, Immunohistochemistry (Frozen) 1:100-1:300
ReactivityHuman, Mouse, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit-IgG
ImmunogenSynthetic peptide / encompassing a sequence within the C-terminus region.
TargetBeta Tubulin 3 / Tuj1
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Beta Tubulin 3 Rabbit Polyclonal Antibody

All lanes: anti-Beta Tubulin 3/ Tuj1 antibody at 1/5000 dilution, Lysates/proteins at 20 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294407 overnight at 4°C.

Beta Tubulin 3 Rabbit Polyclonal Antibody

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294407 anti-Tuj1 antibody (red) at 1:200 and 4°C.

Beta Tubulin 3 Rabbit Polyclonal Antibody

Immunofluorescent analysis. Sample: primary cortical neurons, Tuj1 / Beta Tubulin 3 (orb1294407): 1-300, Anti-rabbit 488: 1-500, Fixed: 4% paraformaldehyde at RT for 20 min.

Beta Tubulin 3 Rabbit Polyclonal Antibody

Immunohistochemical analysis (Frozen sectioned), Sample: Rat brain, Red: Tuj1 antibody (orb1294407), Blue: DAPI.

Beta Tubulin 3 Rabbit Polyclonal Antibody

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Tuj1 with orb1294407 at 1/200.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

Beta Tubulin 3 Rabbit Polyclonal Antibody (orb1294407)

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25 μl
$ 260.00
100 μl
$ 390.00
DispatchUsually dispatched within 5-10 working days
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