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BMI1 Antibody
Description
Images & Validation
−| Tested Applications | FC, ICC, IHC |
|---|---|
| Dilution range | IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 3E3H3 |
| Immunogen | Purified recombinant fragment of human BMI1 expressed in E. Coli. |
| Target | BMI1 |
| Molecular Weight | 37kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
Alternative Names
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Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of BMI1 antibody

Western blot analysis of HEK293 (Lane1) and BMI1-hIgGFc transfected HEK293 (Lane2) cell lysate using BMI1 antibody

Confocal immunofluorescence analysis of Hela cells using BMI1 antibody

IHC-P of rectum cancer tissues using BMI1 antibody

Immunohistochemical analysis of formalin fixed and paraffin-embedded cervical cancer tissues using BMI1 antibody

Western blot analysis of human BMI1 (AA: 1-326) recombinant protein using BMI1 antibody

Flow cytometric analysis of NIH/3T3 cells using BMI1 antibody
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Protocol Information
BMI1 Antibody (orb97390)
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