Bovine Serum Albumin Antibody BSA

SKU: orb2652802

Description

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000-1:10,000
Reactivity	Bovine
Application Notes	Anti-Bovine Serum Albumin (BSA) antibody has been tested by western bot and ELISA and is suitable in dot blot, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 100 µL

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-Bovine Serum Albumin antibody was produced by repeated immunizations with bovine serum albumin.
Purity	Bovine Serum Albumin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Albumin (Bovine Serum). Cross reactivity against Albumin from other tissues and species may occur but has not been specifically determined.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ALB antibody, Albumin antibody, Allergen Bos d 6 antibody, BSA antibody, DKFZp779N1935 antibody, rabbit Anti-BSA, rabbit anti-bovine serum albumin

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ELISA results of purified Rabbit anti-Bovine Serum Albumin Antibody tested against Bovine Serum Albumin. Each well was coated in duplicate with 1.0 µg of the antigen. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n orb347654) and TMB ELISA Peroxidase Substrate (p/n orb348651).

Western Blot of Rabbit Anti-BSA Antibody. Lane 1: Molecular Weight Marker. Lane 2: BSA. Primary Antibody: Rabbit anti-Bovine Serum Albumin at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit Peroxidase conjugated (p/n orb347654) at 1:40000 for 30 min at RT. Blocking: 5% goat serum.

Quick Database Links

UniProt

P02769

RefSeq:AAA51411.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	AAA51411.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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