Recombinant Calponin Antibody

SKU: orb606537

Description

Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.

Research Area

Epigenetics

Images & Validation

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Tested Applications	FACS, IHC-P, WB
Dilution Range	Western blot: 0.5-1ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Reactivity	Human, Rat
Application Notes	Optimal dilution of the recombinant Calponin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rCNN1/832
Immunogen	Recombinant human protein was used as the immunogen for the recombinant Calponin antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC testing of FFPE human uterus stained with recombinant Calponin antibody (clone rCNN1/832). Required HIER: boil tissue sections in 1mM EDTA, pH7.5-8.5, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Calponin antibody (clone rCNN1/832) as confirmation of integrity and purity.

Quick Database Links

UniProt

P51911

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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