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CASP3 Antibody
SKU: orb625436
Featured
Description
Research Area
Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, IP, WB |
|---|---|
| Dilution range | WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:500; IF: 1:20-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | caspase 3, apoptosis-related cysteine peptidase |
| Target | CASP3 |
| Molecular Weight | 32-35 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Disclaimer | For research use only |
Alternative Names
−Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1
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Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using orb625436 (Caspase 3 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).
IP result of anti-Caspase 3 (IP: orb625436, 4 ug; Detection: orb625436 1:300) with NIH/3T3 cells lysate 3440 ug.
HeLa cells were subjected to SDS PAGE followed by western blot with orb625436 (CASP3 antibody) at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded human spleen using orb625436 (CASP3 antibody) at dilution of 1:50.
Quick Database Links
Gene Symbol
CASP3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
Filter by Applications
Filter by Species
Tayebeh Mohtashami et al. Neuroprotective Effects of Platelet-Derived Exosomes in a Rat Model of Methamphetamine-Induced Neurotoxicity Mol Neurobiol ., (2025)
Applications
IHC
Reactivity
Rat
CASP3 Antibody (orb625436)
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