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CD276 Antibody
SKU: orb1925354
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000 |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1,κ |
| Molecular Weight | 57235 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−B7H3
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Immunohistochemical analysis of paraffin-embedded Human tonsil section using CD276 Antibody. Diluted at 1:400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of CD276 Antibody in MCF-7 cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1: 25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Immunohistochemical analysis of CD276 Antibody in 293T (untransfected) cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes: Anti-CD276 Antibody at 1:4000 dilution. Lane 1: 293T-17 whole cell lysate. Lane 2: A-431 whole cell lysate. Lane 3: U-20S whole cell lysate. Lane 4: LNCap whole cell lysate. Lane 5: Hela whole cell lysate. Lane 6: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
CD276 Antibody (orb1925354)
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