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CD276 Antibody
Description
Images & Validation
−| Tested Applications | IHC |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | B7H3/4345 |
| Immunogen | Recombinant fragment (around aa100-300) of human CD276 protein (exact sequence is proprietary) |
| Target | CD276 |
| Source/Expression System | Bioreactor |
| Molecular Weight | B7-H3 isoforms: 57/34/53/57 kDa; glycosylated B7-H3: 90-110 kDa |
| Purification | Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Disclaimer | For research use only |
Alternative Names
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Analysis of Protein Array containing more than 19000 full-length human proteins using CD276 / B7-H3 Monospecific Mouse Monoclonal Antibody (B7H3/4345). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with CD276 / B7-H3 Mouse Monoclonal Antibody (B7H3/4345) at 2 ug/ml. HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min.

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with CD276 / B7-H3 Mouse Monoclonal Antibody (B7H3/4345) at 2 ug/ml. HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min.

SDS-PAGE Analysis of Purified CD276 Mouse Monoclonal Antibody (B7H3/4345). Confirmation of Purity and Integrity of Antibody.
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CD276 Antibody (orb1410558)
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