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CD45RB Antibody

SKU: orb2637916

Description

CD45RB is one of the four major isoforms of CD45. The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. Clone PD7/26 antibody is specific to CD45RB, and stains most leukemias and lymphomas. This CD45RB antibody is often used in a cocktail with clone 2B11, which will bind to all CD45 isoforms.

Images & Validation

Tested ApplicationsIHC-P
Dilution rangeThe prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
ReactivityHuman
Application Notes
Variations in protocols, secondaries and substrates may require the CD45RB antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.PD7/26
ImmunogenHuman peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this CD45RB antibody.
PurificationProtein G purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives1X PBS, pH 7.4
DisclaimerFor research use only

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

CD45RB Antibody

IHC testing of human tonsil (10X) stained with CD45RB antibody (PD7/26).

CD45RB Antibody

IHC testing of human tonsil (20X) stained with PD7/26 mAb.

CD45RB Antibody

SDS-PAGE Analysis of Purified, BSA-Free CD45RB Antibody (clone PD7/26). Confirmation of Integrity and Purity of the Antibody.

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol

CD45RB Antibody (orb2637916)

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7 ml
$ 780.00
DispatchUsually dispatched within 5-10 working days
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