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CD45RB Antibody
SKU: orb2637917
Description
Images & Validation
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| Tested Applications | FACS, IF, IHC-P, WB |
|---|---|
| Dilution range | Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | PD7/26 |
| Immunogen | Human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this CD45RB antibody. |
| Purification | Protein G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 1X PBS, pH 7.4 |
| Disclaimer | For research use only |
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FACS, IF, IHC-P, WB
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Recombinant
Unconjugated
20 μg, 100 μg
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IHC testing of human tonsil (10X) stained with CD45RB antibody (PD7/26).
IHC testing of human tonsil (20X) stained with PD7/26 mAb.
Western blot testing of Daudi cell lysate with CD45RB antibody (clone PD7/26). Predicted molecular weight: ~147/180~220kDa (unmodified/glycosylated).
SDS-PAGE Analysis of Purified, BSA-Free CD45RB Antibody (clone PD7/26). Confirmation of Integrity and Purity of the Antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
CD45RB Antibody (orb2637917)
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