CD86 Rabbit mAb Antibody

SKU: orb1951832

Description

Images & Validation

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Item 1 of 6

Tested Applications	FC, ICC, IHC, IP, WB
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Recombinant Antibody
Host	Rabbit
Clonality	Monoclonal
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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All Lanes: CD86 Antibody at 1/1000 dilution. Lane 1: HeLa. Lane 2: Jurkat. Lane 3: MCF-7. Lane 4: SH-SY5Y. (Proteins at 20 ug per lane). Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 70 kDa, Observed band size: 70 kDa.

All Lanes: CD86 Antibody at 1/1000 dilution. Lane 1: Mouse heart. Lane 2: Mouse spleen. Lane 3: Mouse lung. Lane 4: Rat heart. Lane 5: Rat liver. (Proteins at 20 ug per lane). Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 70 kDa, Observed band size: 70 kDa.

All Lanes: CD86 Antibody at 1/1000 dilution. Lane 1: Raji cell lysate. Proteins at 20 ug per lane, Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 70 kDa, Observed band size: 70 kDa.

Immunofluorescent analysis of K562 cells, using CD86 Antibody. 4% polyformaldehyde fixed. 0.1% tritonX-100 permeable. DAPI stains the nucleus.

Immunofluorescent analysis using the Antibody at 1:50 dilution. 4% polyformaldehyde fixed. 0.1% tritonX-100 permeable. DAPI stains the nucleus.

Immunohistochemical analysis of paraffin-embedded human tonsil, using CD86 Antibody. Tris-EDTA Buffer (pH9.0).

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UniProt

P42081

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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ICC

Immunocytochemistry

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Immunoprecipitation

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