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CD86 Recombinant Protein
Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0. 04 - 0. 2 ng/ml. Measured by its binding ability in a functional ELISA. Immobilized rhB7-2 / CD86 Fc Chimera at 2μg/mL (100μL/well) can bind Biotinylated rh CTLA4, His. Avi Tag with a linear range of 0. 1 - 1μg/mL. |
| Target | CD86 |
| Tag | C-Fc Tag |
| Molecular Weight | 51.5 kDa |
| Protein Sequence | Leu 26 - Pro 247 |
| Purity | >95% as determined by SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant CD86 Antibody [orb606385]
ELISA, FACS, IF, IHC-P, WB
Human
Rabbit
Recombinant
Unconjugated
100 μg, 20 μgHuman B7-2 Protein, mFc-His Tag [orb689395]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 52.4 kDa after removal of the signal peptide.
Mammalian
10 μg, 100 μg, 50 μgRecombinant Human B7-2 Protein (Biotin) [orb2993754]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 28.4 KDa. Observed: 55-80 KDa, reducing conditions
20 μg, 100 μgRecombinant Cynomolgus B7-2 Protein [orb2994271]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 52.5 KDa. Observed: 70-100 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Mouse B7-2 Protein [orb2994287]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 25.9 KDa. Observed: 35-85 KDa, reducing conditions
10 μg, 50 μg, 1 mg, 500 μg

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The purity of rh CD86 Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
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CD86 Recombinant Protein (orb1228136)
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