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COL2A1 Antibody
SKU: orb1259434
Description
Research Area
Cell Biology, Signal Transduction
Images & Validation
−Item 1 of 1
| Tested Applications | IF, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 873-1072 of human COL2A1 (NP_149162.2). |
| Target | COL2A1 |
| Molecular Weight | Observed: 141kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Collagen alpha-1(II) chain, Alpha-1 type II collagen, Collagen alpha-1(II) chain, Chondrocalcin, COL2A1
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Western blot analysis of extracts of mouse liver, using COL2A1 antibody (orb1259434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Quick Database Links
Gene Symbol
COL2A1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
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Yuan Lin 1, Xinpeng Zheng 1, Xiaolei Chen 1, Yue Wang Sp2 Transcription Factor Alleviates Chondrocyte Loss in Osteoarthritis by Repressing the DVL1-Dependent Wnt/β-Catenin Signaling Pathway J Gene Med, (2025)
Applications
WB
Reactivity
Mouse
COL2A1 Antibody (orb1259434)
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