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Collagen III Rabbit Polyclonal Antibody
SKU: orb304041
Description
Images & Validation
−Item 1 of 6
| Tested Applications | ELISA, IHC-P |
|---|---|
| Dilution Range | IHC-P: 1:100-500 |
| Reactivity | Bovine, Canine, Gallus, Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Collagen III. Please contact us for the exact immunogen sequence. The peptide is available as orb374884. |
| Target | Collagen III |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti COL3A1 antibody, anti Collagen alpha 1(III) chain antibody, anti Collagen III antibody, anti Collagen III alpha 1 chain precursor antibody, anti Collagen III alpha 1 polypeptide antibody, anti Collagen type III alpha antibody, anti Collagen type III alpha 1 antibody, anti Collagen type III alpha 1 antibody, anti Fetal collagen antibody, anti Type III collagen antibody
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Immunohistochemical staining of paraffin embedded rat pancreas tissue using Collagen III antibody (2.5 ug/ml)
IHC-P image of human thyroid cancer tissue using Collagen III antibody (2.5 ug/ml)
IHC-P staining of rat pancreas tissue using Collagen III antibody (2.5 ug/ml)
Immunohistochemical staining of human thyroid cancer tissue using anti-Collagen III (2.5 ug/ml)
Immunohistochemical staining of human endometrial cancer tissue using anti-Collagen III (2.5 ug/ml)
IHC-P staining of human endometrial cancer tissue using anti-Collagen III (2.5 ug/ml)
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Collagen III Rabbit Polyclonal Antibody (orb304041)
- 4.0
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- 4 stars
Useful Collagen III Antibody from Biorbyt. Alkaline antigen retrieval (pH 9.0) caused more background staining. I used citrate pH 6.0 to reduce background staining. I increased the BSA that I normally use from 1% to 3%. There was a nice response. I can say that I got a better response than I expected in my trying. I use these types of collagen antibodies in wound healing and scar studies. Also, It can be used for the state of inflammatory processes of any inflammation or tumor stroma. It does a light nonspecific staining.
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