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DC-SIGN/CD209 Antibody
SKU: orb318734
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, FC, IF, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:128000, WB: 0.1-0.3 μg/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | DC-SIGN / CD209 |
| Molecular Weight | 45.8; 43.3; 45.0; 30.5; 41.0; 35.2; 27.4 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti CD209 antibody, anti CD209 molecule antibody, anti CDSIGN antibody, anti CLEC4L antibody, anti DC-SIGN antibody, anti DC-SIGN1 antibody, anti C-type lectin domain family 4 member L antibody, anti C-type lectin domain family 4, member L antibody, anti CD209 antigen antibody, anti HIV gpl20-binding protein antibody, anti dendritic cell-specific ICAM-3-grabbing non-integrin 1 antibody, anti dendritic cell-spe antibody
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DC-SIGN / CD209 Reference Antibody [orb1806229]
ELISA, FA, FACS, In vivo
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1 µg/ml staining of K562 (A) and (2 ug/ml) THP-1 (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
6 µg/ml staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
Flow cytometric analysis of paraformaldehyde fixed THP1 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunofluorescence analysis of paraformaldehyde fixed THP-1 immobilized on Shi-fix™ plus cover-slips. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DC-SIGN/CD209 Antibody (orb318734)
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