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DNAJB1 Antibody
SKU: orb1274448
Featured
Description
Images & Validation
−Item 1 of 5
| Tested Applications | IF, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human DNAJB1 (NP_006136.1). |
| Target | DNAJB1 |
| Molecular Weight | Observed: 39kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, Heat shock 40 kDa protein 1, HSP40, Heat shock protein 40, Human DnaJ protein 1, hDj-1, DNAJB1, DNAJ1, HDJ1, HSPF1
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Western blot analysis of extracts of various cell lines, using DNAJB1 antibody (orb1274448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Western blot analysis of extracts from normal (control) and DNAJB1 knockout (KO) 293T cells, using DNAJB1 antibody (orb1274448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of U2OS cells using DNAJB1 antibody (orb1274448). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using DNAJB1 antibody (orb1274448) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using DNAJB1 antibody (orb1274448) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
DNAJB1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
DNAJB1 Antibody (orb1274448)
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