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EGFR mutant

SKU: orb197814
FeaturedFeatured Product

Description

Mouse monoclonal antibody to EGFR mutant (L858R)

Images & Validation

Tested ApplicationsFC, IHC
Dilution rangeIHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
ReactivityHuman

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1
Clone No.5G9B5
ImmunogenPurified recombinant fragment of human EGFR mutant (AA: 693-893) expressed in E. Coli.
TargetEGFR mutant
Molecular Weight175kDa
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesAscitic fluid containing 0.03% sodium azide.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

ERBB; HER1; mENA; ERBB1; PIG61

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EGFR mutant

Western blot analysis of SPC-A-1 (Lane 1), A549 (Lane 2), HepG2 (Lane 3) MCF-7 (Lane 4) cell lysate using EGFR mutant antibody

EGFR mutant

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of EGFR mutant antibody

EGFR mutant

Flow cytometric analysis of HepG2 cells lysates using EGFR mutant antibody

EGFR mutant

Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using EGFR mutant antibody

EGFR mutant

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rectum cancer tissues using EGFR mutant antibody

EGFR mutant

Western blot analysis of human EGFR mutant using EGFR mutant antibody

EGFR mutant

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using EGFR mutant antibody

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol

EGFR mutant (orb197814)

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Available Sizes

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100 μl
$ 350.00
DispatchUsually dispatched within 5-10 working days
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