EpCAM Recombinant Rabbit Monoclonal Antibody

SKU: orb1816732

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Description

Research Area

Cancer Research, Epi / Endothelial, Tumor Biology, Varscular System

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human
Predicted Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	C4A12
Immunogen	A synthesized peptide derived from human EpCAM (64-100/314aa)
Target	EPCAM
Molecular Weight	35 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS, Glycerol, rAlbumin.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Epithelial Specific Antigen; ESA; epithelial cell adhesion molecule; Tumor-associated calcium signal transducer 1EPCAM; EGP; KSA; M4S1; MH99; MK-1; 17-1A; CD326; EGP-2; EGP34; EGP40; KS1/4; MIC18; MOC31; TROP1; 323/A3; CO-17A; Ep-CAM; HEA125; hEGP-2; CO17-1A; GA733-2; TACST-1; TACSTD1; ESA; KSA; M4S1; MK-1; DIAR5; TROP1; EGP314; HNPCC8; EPCAM_HUMAN.

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EpCAM Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (EpCAM) Monoclonal Antibody, Unconjugated (orb1816732) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (EpCAM) Monoclonal Antibody, Unconjugated (orb1816732) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (EpCAM) Monoclonal Antibody, Unconjugated (orb1816732) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

EPCAM

UniProt

P16422

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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