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Estrogen Receptor alpha Antibody
SKU: orb639637
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 4
| Tested Applications | FACS, IHC-P |
|---|---|
| Dilution Range | Flow cytometry: 1-2ug/10^6 cells in 0.1ml,Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2b, kappa |
| Clone No. | ESR1/3559 |
| Immunogen | A recombinant human partial protein (amino acids 129-312) was used as the immunogen for this Estrogen Receptor alpha antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Flow cytometry testing of permeabilized human MCF7 cells with Estrogen Receptor alpha antibody (clone ESR1/3559); Red=isotype control, Blue= Estrogen Receptor alpha antibody.
IHC staining of FFPE human breast carcinoma with Estrogen Receptor alpha antibody (clone ESR1/3559). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Estrogen Receptor alpha antibody (clone ESR1/3559). These results demonstrate the foremost specificity of the ESR1/3559 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Estrogen Receptor alpha antibody (clone ESR1/3559) as confirmation of integrity and purity.
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
Estrogen Receptor alpha Antibody (orb639637)
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