F(ab')2 Goat IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

SKU: orb348127

Description

F(ab')2 Goat IgG (H&L) antibody (RPE)

Images & Validation

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Item 1 of 2

Tested Applications	DOT, FC, IF, WB
Dilution range	FC: 1:100 - 1:250, IF: 1:100 - 1:250
Reactivity	Goat
Application Notes	F(ab')2 Anti-Goat IgG Phycoerythrin Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Read more...

Key Properties

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Antibody Type	Secondary Antibody
Host	Donkey
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Anti-Goat IgG was produced by repeated immunization with goat IgG whole molecule in donkey.
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, or Rat Serum Proteins.
Conjugation	RPE

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.05% (w/v) Sodium Azide
Concentration	1.032 mg/ml
Disclaimer	For research use only

Alternative Names

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Donkey F(ab')2 Anti-Goat IgG Antibody Phycoerythrin Conjugation, Donkey Fab2 Anti-Goat IgG PE Conjugated Antibody

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F(ab')2 Goat IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

Dot Blot of F(ab')2 Donkey anti-Goat IgG Phycoerythrin Conjugated Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, & Rt serum proteins antibody. Antigen: Goat IgG. Load: 100 ng, 33.3 ng, 11.1 ng, 3.7 ng, or 1.23 ng as indicated. Primary antibody: N/A. Secondary antibody: F(ab')2 Donkey anti-Goat IgG Phycoerythrin Conjugated Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, & Rt serum proteins antibody at 1:1000 for 60 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637) for 60 min at RT.

Dot Blot results of Donkey F(ab')2 Anti-Goat IgG Antibody Phycoerythrin Conjugated. Dots are Goat IgG at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: none. Secondary Antibody: Donkey F(ab')2 Anti-Goat IgG Antibody RPE at 1 µg/ml for 1hr at RT.

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Western Blot (IB, immunoblot)

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Immunofluorescence

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DOT

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