F(ab')2 Rabbit IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

SKU: orb348316

Description

F(ab')2 Rabbit IgG (H&L) antibody (RPE)

Images & Validation

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Tested Applications	FC, IF, WB
Dilution range	FC: 1:100 - 1:250, IF: 1:100 - 1:250
Reactivity	Rabbit
Application Notes	F(ab')2 Anti-Rabbit IgG Phycoerythrin Antibody has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Read more...

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Rabbit IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Conjugation	RPE

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

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Goat F(ab')2 Anti-Rabbit IgG Phycoerythrin Conjugated Pre-Adsorbed Antibody, Goat Fab2 Anti-Rabbit IgG Antibody PE Conjugation

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunofluorescence