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GAPDH Rabbit Polyclonal Antibody
SKU: orb1294414
Description
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | Western Blot 1:5000-1:10000, Immunofluorescence 1:200-1:500, Immunohistochemistry (Paraffin) 1:400-1:600 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
| Target | GAPDH |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Disclaimer | For research use only |
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Anti-GAPDH antibody - 1/5000 dilution, Lysates at 20 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294414 overnight at 4°C.
Anti-GAPDH antibody - 1/5000 dilution, Lysates at 20 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294414 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294414 anti-GAPDH antibody (red) at 1:200 and 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294414 anti-GAPDH antibody (red) at 1:200 and 4°C.
Immunohistochemical analysis of paraffin embedded mouse testis labeling GAPDH with orb1294414 at 1/400.
Quick Database Links
Gene Symbol
GAPDH
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
GAPDH Rabbit Polyclonal Antibody (orb1294414)
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