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GC Antibody
SKU: orb1257373
Featured
Description
Research Area
Cancer Biology, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 324-493 of human GC (NP_001191236.1). |
| Target | GC |
| Molecular Weight | Observed: 60kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−VDBP, vitamin D-binding protein/group specific component, vitamin D-binding protein, vitamin D-binding alpha-globulin, vitamin D binding protein, VDBP, VDBG, DBP/GC, DBP, group-specific component (vitamin D binding protein), GC, GRD3, HEL-S-51
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Western blot analysis of extracts of various cell lines, using GC antibody (orb1257373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.
Immunofluorescence analysis of U2OS cells using GC antibody (orb1257373). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
GC
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
GC Antibody (orb1257373)
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