GCLM Rabbit Polyclonal Antibody

SKU: orb500495

Description

Research Area

Amino Acid Metabolism, Cancer Research, Cell Biology, Drug Metabolism, Energy Metabolism, Glutamate, Metabolism, Mitochondrial, Mitochondrial Markers, Neuroscience, Oxidative Stress

Images & Validation

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Item 1 of 1

Tested Applications	IF, IHC-Fr, IHC-P
Dilution Range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Rat
Predicted Reactivity	Human, Mouse

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human GCLM (121-220/274aa)
Target	GCLM
Molecular Weight	31 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Glutamate-Cysteine Ligase Modifier Subunit; gamma-Glutamylcysteine Synthetase Regulatory Subunit; Glutamate--Cysteine Ligase Modifier Subunit; gamma-ECS Regulatory Subunit; GCS Light Chain; GLCLR; Glutamate-Cysteine Ligase(gamma-Glutamylcysteine Synthetase), Regulatory(30.8kD); Glutamate-Cysteine Ligase Modifier Subunit delta2 Alternative Splicing; Glutamate--Cysteine Ligase Regulatory Subunit; Glutamate-Cysteine Ligase Regulatory Protein; Glutamate-Cysteine Ligase, Modifier Subunit; gamma-Glutamylcysteine Synthetase; GSC Light Chain; GSH0_HUMAN;

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Paraformaldehyde-fixed, paraffin embedded (rat liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GCLM) Polyclonal Antibody, Unconjugated (orb500495) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

GCLM

UniProt

P48507

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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