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Glypican 3 Recombinant Rabbit Monoclonal Antibody
SKU: orb1152010
Featured
Description
Research Area
Cancer Research, Cell Signaling, Developmental Biology, Heparan Sulfate ProteoGlycan, Tumor Biomarkers
Images & Validation
−Item 1 of 3
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB=1:500-2000, Flow-Cyt=1:20-100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Immunogen | A synthesized peptide derived from human Glypican 3 (1-250/580aa) |
| Target | GPC3 |
| Molecular Weight | 65 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−GPC3; glypican3; glypican-3; Intestinal protein OCI-5; GTR2-2; MXR7.Intestinal protein OCI-5; GPC3_HUMAN.
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: HepG2, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 66 kDa, Observed MW: 66 kDa.
Cell line: HepG2, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152010.
Cell line: HepG2, Fixative: 4% Paraformaldehyde, Permeabilization: 90% methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1152010.
Quick Database Links
Gene Symbol
GPC3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
Glypican 3 Recombinant Rabbit Monoclonal Antibody (orb1152010)
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